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dsmz germany  (DSMZ)


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    DSMZ dsmz germany
    Dsmz Germany, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsmz+germany/293/pmc12920735-220-6-6
    Average 96 stars, based on 371 article reviews
    dsmz germany - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: Cell-based and isoform-selective G protein-coupled receptor kinase assays for comprehensive inhibitor evaluation.
    Article Snippet: Cell lines and culture HEK293 cells were originally obtained from DSMZ Germany (ACC 305).

    Article Title: Histone deacetylase inhibitors promote breast cancer metastasis by elevating NEDD9 expression
    Article Snippet: CAL-51 was got from DSMZ (Germany).

    Article Title: Rapid elucidation of agonist-driven regulation of the neurokinin 1 receptor using a GPCR phosphorylation immunoassay.
    Article Snippet: 94 HEK293 cells were originally purchased from DSMZ Germany (ACC 305).

    Article Title: Hybridization into a Bitopic Ligand Increased Muscarinic Receptor Activation for Isopilocarpine but Not for Pilocarpine Derivatives.
    Article Snippet: Pilocarpine (1), a secondary metabolite of several Pilocarpus species, is a therapeutically used partial agonist of muscarinic acetylcholine receptors (mAChRs).. The available pharmacological data and structure−activity relationships do not provide comparable data for all five receptor subtypes.. In this study, pilocarpine (1), its epimer isopilocarpine (2), racemic analogues pilosinine (3) and desmethyl pilosinine (4), and the respective hybrid ligands with a naphmethonium fragment (5-C6 to 8-C6) were synthesized and analyzed in mini-G nano-BRET assays at the five mAChRs.

    Article Title: Cell-based and isoform-selective G protein-coupled receptor kinase assays for comprehensive inhibitor evaluation
    Article Snippet: HEK293 cells were originally obtained from DSMZ Germany (ACC 305).

    Article Title: Selective killing of plasma cell clones using splice-switching oligonucleotides targeting immunoglobulin variable exons
    Article Snippet: The human myeloma cell lines SK-MM-2 from DSMZ (Germany) and RPMI 8226 from ATTC (USA) were cultured in RPMI 1640 medium in the presence of 10% fetal calf serum (FCS).

    Cell Culture:

    Article Title: High-fat diet impairs microbial metabolite production and aggravates influenza A infection
    Article Snippet: .. HEK293 cells were obtained from DSMZ Germany (ACC 305) and cultured at 37 °C with 5% CO2 in DMEM (Sigma-Aldrich, Germany), supplemented with 10% FCS and 1% penicillin and streptomycin. ..

    Article Title: High-fat diet impairs microbial metabolite production and aggravates influenza A infection.
    Article Snippet: .. HEK293 cells were obtained from DSMZ Germany (ACC 305) and cultured at 37 °C with 5% CO2 in DMEM (Sigma-Aldrich, Germany), supplemented with 10% FCS and 1% penicillin and streptomycin. ..



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    DSMZ germany acc 115 rrid cvcl 0031 t47d dsmz
    Figure 5. Functional in vitro validation of SOX2 C terminus-imposed translational adaptations (A) Relative changes in glucose uptake in response to SOX2 (fragment) induction in <t>T47D</t> cells. Indicated are MFI shifts at l = 488 nm (D mean 488 nm) reflecting SOX2 (fragment)-induced changes to the incorporation rate of glucose uptake reporter 2-NBDG. The endogenous uptake rate into DOX-exposed but empty control cells is set to baseline. Dots indicate mean intensity shift in 4 independent experiments. (B) Representative example of a metabolic Seahorse analysis indicating aggravated sugar consumption (followed as extracellular acidification rate [ECAR]) in starved T47D cells expressing either full-length SOX2 (1–317, red) or an NES/C-terminal segment thereof (180–317, green) vs. equally treated empty control cells (black) or T47D cells expressing the SOX2 N terminus (1–179, blue). Dots reflect means of 4 or more technical replicates.
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    Figure 5. Functional in vitro validation of SOX2 C terminus-imposed translational adaptations (A) Relative changes in glucose uptake in response to SOX2 (fragment) induction in T47D cells. Indicated are MFI shifts at l = 488 nm (D mean 488 nm) reflecting SOX2 (fragment)-induced changes to the incorporation rate of glucose uptake reporter 2-NBDG. The endogenous uptake rate into DOX-exposed but empty control cells is set to baseline. Dots indicate mean intensity shift in 4 independent experiments. (B) Representative example of a metabolic Seahorse analysis indicating aggravated sugar consumption (followed as extracellular acidification rate [ECAR]) in starved T47D cells expressing either full-length SOX2 (1–317, red) or an NES/C-terminal segment thereof (180–317, green) vs. equally treated empty control cells (black) or T47D cells expressing the SOX2 N terminus (1–179, blue). Dots reflect means of 4 or more technical replicates.

    Journal: Cell reports

    Article Title: Nuclear and cytosolic fractions of SOX2 synergize as transcriptional and translational co-regulators of cell fate.

    doi: 10.1016/j.celrep.2024.114807

    Figure Lengend Snippet: Figure 5. Functional in vitro validation of SOX2 C terminus-imposed translational adaptations (A) Relative changes in glucose uptake in response to SOX2 (fragment) induction in T47D cells. Indicated are MFI shifts at l = 488 nm (D mean 488 nm) reflecting SOX2 (fragment)-induced changes to the incorporation rate of glucose uptake reporter 2-NBDG. The endogenous uptake rate into DOX-exposed but empty control cells is set to baseline. Dots indicate mean intensity shift in 4 independent experiments. (B) Representative example of a metabolic Seahorse analysis indicating aggravated sugar consumption (followed as extracellular acidification rate [ECAR]) in starved T47D cells expressing either full-length SOX2 (1–317, red) or an NES/C-terminal segment thereof (180–317, green) vs. equally treated empty control cells (black) or T47D cells expressing the SOX2 N terminus (1–179, blue). Dots reflect means of 4 or more technical replicates.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: cell lines MCF7 DSMZ, Braunschweig, Germany ACC-115 RRID:CVCL_0031 T47D DSMZ, Braunschweig, Germany ACC-739 RRID:CVCL_0553 MDA-MB468 DSMZ, Braunschweig, Germany ACC-738 RRID:CVCL_0419 OVCAR3 ATCC, Manassas, VA, USA, HTP-161 RRID:CVCL_0465 U373 ATCC, Manassas, VA, USA, kindly provided by L. Mariani (Head of Neurosurgery, University Hospital Basel, Switzerland) HTB-017 RRID:CVCL_2219 LN215 kindly provided by L. Mariani RRID: CVCL_3954 LN229 ATCC, Manassas, VA, USA, kindly provided by L. Mariani CRL-2611 RRID:CVCL_0393 LN319 kindly provided by L. Mariani RRID: CVCL_3958 LN401 kindly provided by L. Mariani RRID, CVCL_6840 LN405 kindly provided by L. Mariani RRID, CVCL_1379 U87 ATCC, Manassas, VA, USA, kindly provided by L. Mariani HTB-14 RRID:CVCL_0022 HEK (i.e., HEK 293T) DSMZ, Braunschweig, Germany ACC-635 RRID:CVCL_0063 MEF (genetically modified to carry an Oct4 promotor-GFP transgene, OG2, see ref.76) kindly provided by H.R.

    Techniques: Functional Assay, In Vitro, Biomarker Discovery, Control, Expressing

    Figure 6. A disease-linked human mutant abolishes the translational role of SOX2 (A) Western blot analyses verify inducibility of the indicated SOX2 variants expressed as mCherry-fusions in T47D. The nucleocytoplasmic transport mutants T116A and T118A, DNA non-binding M49G, and disease-associated SOX2 variants L97P, G130A, and A191T were investigated. Anti-mCherry staining (top) confirms robust induction relative to wild-type transduced cells (blue). Anti-pRPS6(235/236) staining (center) indicates SOX2-imposed changes for all constructs but SOX2(L97P) (red). Anti-RPS6 staining (control, bottom).

    Journal: Cell reports

    Article Title: Nuclear and cytosolic fractions of SOX2 synergize as transcriptional and translational co-regulators of cell fate.

    doi: 10.1016/j.celrep.2024.114807

    Figure Lengend Snippet: Figure 6. A disease-linked human mutant abolishes the translational role of SOX2 (A) Western blot analyses verify inducibility of the indicated SOX2 variants expressed as mCherry-fusions in T47D. The nucleocytoplasmic transport mutants T116A and T118A, DNA non-binding M49G, and disease-associated SOX2 variants L97P, G130A, and A191T were investigated. Anti-mCherry staining (top) confirms robust induction relative to wild-type transduced cells (blue). Anti-pRPS6(235/236) staining (center) indicates SOX2-imposed changes for all constructs but SOX2(L97P) (red). Anti-RPS6 staining (control, bottom).

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: cell lines MCF7 DSMZ, Braunschweig, Germany ACC-115 RRID:CVCL_0031 T47D DSMZ, Braunschweig, Germany ACC-739 RRID:CVCL_0553 MDA-MB468 DSMZ, Braunschweig, Germany ACC-738 RRID:CVCL_0419 OVCAR3 ATCC, Manassas, VA, USA, HTP-161 RRID:CVCL_0465 U373 ATCC, Manassas, VA, USA, kindly provided by L. Mariani (Head of Neurosurgery, University Hospital Basel, Switzerland) HTB-017 RRID:CVCL_2219 LN215 kindly provided by L. Mariani RRID: CVCL_3954 LN229 ATCC, Manassas, VA, USA, kindly provided by L. Mariani CRL-2611 RRID:CVCL_0393 LN319 kindly provided by L. Mariani RRID: CVCL_3958 LN401 kindly provided by L. Mariani RRID, CVCL_6840 LN405 kindly provided by L. Mariani RRID, CVCL_1379 U87 ATCC, Manassas, VA, USA, kindly provided by L. Mariani HTB-14 RRID:CVCL_0022 HEK (i.e., HEK 293T) DSMZ, Braunschweig, Germany ACC-635 RRID:CVCL_0063 MEF (genetically modified to carry an Oct4 promotor-GFP transgene, OG2, see ref.76) kindly provided by H.R.

    Techniques: Mutagenesis, Western Blot, Binding Assay, Staining, Construct, Control